Supplemental Movies

Three supplemental movies in .MP4 (MPEG-4) format. Right-click on the links and choose "Open in a New Window" for easier viewing. This will open the movie in a separate window, leaving the captions on the screen. If possible, set your viewer to loop the video clip.

Files in this Data Supplement:

• Fig. 17 - The clip shows the flow of blood through the afferent filamental artery (AFA) and a series of afferent lamellar arterioles (ALA) leading into lamellae of a single filament in the gill of the North American eel, Anguilla rostrata. The field of view is approximately 300 microns, and the movie is in real time. The flowing objects within the AFA, ALA, and lamellae are erythrocytes and the flow within the lamellae is broken up into many channels around pillar cells, which appear as blank spaces as the erythrocytes flow around them. Fish were anesthetized in 0.1g/l MS222 in sea water and placed, ventral side up, in sufficient aerated sea water to cover the body (containing 0.025g/l MS222).   The operculae were cut along the ventral aspect in order to expose the gills and to immobilize the fish (via pins to the underlying paraffin base).   Gill blood flow was visualized through a 20X water-immersion lens on an Olympus BX30 reflected light microscope (with polarizing filters) and recorded by an Optronics DEI-750 video camera system, which output was connected via a Dazzle A/D converter to a Macintosh iBook G3 running iMovie.
• Fig. 18 - The clip shows the flow of blood through a single lamella in the gill of the North American eel, Anguilla rostrata. The field of view is approximately 300 microns, and the movie is in real time. The flowing objects within the lamella are erythrocytes and the flow is broken up into many channels around pillar cells, which appear as blank spaces as the erythrocytes flow around them. Fish were anesthetized in 0.1g/l MS222 in sea water and placed, ventral side up, in sufficient aerated sea water to cover the body (containing 0.025g/l MS222).   The operculae were cut along the ventral aspect in order to expose the gills and to immobilize the fish (via pins to the underlying paraffin base).   Gill blood flow was visualized through a 20X water-immersion lens on an Olympus BX30 reflected light microscope (with polarizing filters) and recorded by an Optronics DEI-750 video camera system, which output was connected via a Dazzle A/D converter to a Macintosh iBook G3 running iMovie.
• Fig. 38 - The clip shows the flow of blood through the afferent filamental artery and a series of afferent lamellar arterioles leading into lamellae of a single filament in the gill of the longhorn sculpin Myoxocephalus octodecimspinosus. The field of view is approximately 200 microns, and the movie is in real time. The flowing objects within the AFA and outer marginal chanels are erythrocytes. Preliminary cardiovascular studies had shown that ET (human ET-1) produced a concentration-dependent (1, 10, 100, 333, 1000 pmol/kg) increase in gill vascular resistance, so a maximal dose of ET-1 (1000 pmol/kg) was infused into the ventral aorta ten seconds before the start of the clip. Note the nearly complete cessation of flow in the AFA after an additional 15 seconds. Fish were anesthetized in 0.1g/l MS222 in sea water and placed, ventral side up, in sufficient aerated sea water to cover the body (containing 0.025g/l MS222).   The operculae were cut along the ventral aspect in order to expose the gills and to immobilize the fish (via pins to the underlying paraffin base).   Gill blood flow was visualized through a 20X water-immersion lens on an Olympus BX30 reflected light microscope (with polarizing filters) and recorded by an Optronics DEI-750 video camera system, which output was connected via a Dazzle A/D converter to a Macintosh iBook G3 running iMovie.